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BIOM8001 - Applied Separation Technology

Title:Applied Separation Technology
Long Title:Applied Separation Technology
Module Code:BIOM8001
Duration:1 Semester
Credits: 5
NFQ Level:Advanced
Field of Study: Chemistry
Valid From: Semester 1 - 2012/13 ( September 2012 )
Module Delivered in 1 programme(s)
Module Coordinator: Donagh OMahony
Module Description: This module describes the various strategies available for the separation of gaseous, liquid and solid forms.
Learning Outcomes
On successful completion of this module the learner will be able to:
LO1 Describe appropriate methods of evaporation and distillation for the extraction of constituents from plant and other biological materials.
LO2 Critically discuss the various methods of particules sedimentation and centrifugation for the preparation of extracts.
LO3 Distinguish between the various methods of solvent extraction for aqueous and oil soluble constituents.
LO4 Evaluate current methods of fractionation and isolation of bioactive compounds.
LO5 Select different chromatographic protocols for the separation of components and clean-up of analytes.
Pre-requisite learning
Module Recommendations

This is prior learning (or a practical skill) that is strongly recommended before enrolment in this module. You may enrol in this module if you have not acquired the recommended learning but you will have considerable difficulty in passing (i.e. achieving the learning outcomes of) the module. While the prior learning is expressed as named MTU module(s) it also allows for learning (in another module or modules) which is equivalent to the learning specified in the named module(s).

Incompatible Modules
These are modules which have learning outcomes that are too similar to the learning outcomes of this module. You may not earn additional credit for the same learning and therefore you may not enrol in this module if you have successfully completed any modules in the incompatible list.
No incompatible modules listed
Co-requisite Modules
No Co-requisite modules listed

This is prior learning (or a practical skill) that is mandatory before enrolment in this module is allowed. You may not enrol on this module if you have not acquired the learning specified in this section.

No requirements listed

Module Content & Assessment

Indicative Content
Separation Technologies
Separation by gravity (batch and continuous), Sedimentation, Centrifugation, Centrifugal force, Solid phase, Liquid phase, Types of centrifuge, Ultracentrifugation.
Distillation, Dehydration and Evaporation
Distillation. Evaporation. Drying, roller, spray, freeze and fluidised bed, Lyophilisation, Crystallisation, Microwave drying.
Theory of filtration, Batch filters,Continuous filters, Types of filters, Cross flow filtration. Membrane Processes; Reverse osmosis, Ultrafiltration, Liquid membranes and Nanofiltration, Filter characterisation.
Solvent Extraction
Liquid-liquid extraction, Principles of solubility in solutions, Solvent extraction equilibria, Two-phase aqueous extraction, Supercritical fluid extraction, Development of industrial extraction protocols, Extraction of organic compounds, Solvent recovery, Solvent extraction for recovery of waste.
Chromatographic methods
Principles of chromatography, Adsorption chromatography, Gel filtration, Ion exchange, Affinity Chromatography, Thin Layer Chromatography,High Performance Liquid Chromatography, Reverse Phase Chromatography, Gas Chromatography, Liquid Chromatography, Chromatographic-Mass Spectrometric methods.
Assessment Breakdown%
Course Work100.00%
Course Work
Assessment Type Assessment Description Outcome addressed % of total Assessment Date
Essay on methods of separation applied to a specific product. 1,2,3,4 30.0 Week 9
Practical/Skills Evaluation experimental laboratory work and interpretation of data 1,2,3,4,5 30.0 Every Second Week
Short Answer Questions Theory Assessment 1,2,3,4,5 40.0 Sem End
No End of Module Formal Examination
Reassessment Requirement
Repeat examination
Reassessment of this module will consist of a repeat examination. It is possible that there will also be a requirement to be reassessed in a coursework element.

The institute reserves the right to alter the nature and timings of assessment


Module Workload

Workload: Full Time
Workload Type Workload Description Hours Frequency Average Weekly Learner Workload
Lecture Theory 3.0 Every Week 3.00
Lab Practical 2.0 Every Second Week 1.00
Independent & Directed Learning (Non-contact) Self directed study 3.0 Every Week 3.00
Total Hours 8.00
Total Weekly Learner Workload 7.00
Total Weekly Contact Hours 4.00
This module has no Part Time workload.

Module Resources

Recommended Book Resources
  • Sutherland, K. 2006, A-Z of Filtration and Related Separations, Elsevier Science Oxford [ISBN: 13-9781856174077]
  • Harold M. McNair, James M. Miller, 2009, Basic Gas Chromatography, Wiley-Blackwell [ISBN: 0470439548]
  • Veronika R. Meyer 2010, Practical High-performance Liquid Chromatography, Wiley-Blackwell [ISBN: 0470682175]
Supplementary Book Resources
  • Augustijns, P. and Brewster, M. 2007, Solvent systems and their selection in pharmaceutics and biopharmaceutics, Springer-Verlag New York Inc. [ISBN: 13-978-0387691497]
  • Graham, J.M. and Rickwood, D. 2001, Biological Centrifugation, Bios Scientific Publishers Ltd. Oxford [ISBN: 13-9781859960370]
  • Ardrey, R.E. 2003, Liquid Chromatography-Mass Spectrometry: An Introduction, Wiley [ISBN: 0471498017]
  • Theodore H. Meltzer, Maik W. Jornitz 1998, Filtration in the biopharmaceutical industry, Marcel Dekker New York [ISBN: 0824798961]
This module does not have any article/paper resources
This module does not have any other resources

Module Delivered in

Programme Code Programme Semester Delivery
CR_SHERB_8 Bachelor of Science (Honours) in Herbal Science 7 Mandatory

Cork Institute of Technology
Rossa Avenue, Bishopstown, Cork

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