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BIOT7013 - Upstream Bioprocessing

Title:Upstream Bioprocessing
Long Title:Upstream Bioprocessing
Module Code:BIOT7013
Duration:1 Semester
Credits: 5
NFQ Level:Intermediate
Field of Study: Biotechnology
Valid From: Semester 1 - 2017/18 ( September 2017 )
Module Delivered in 5 programme(s)
Module Coordinator: Brigid Lucey
Module Author: Rosemary Rea
Module Description: This module is designed to familarise students with the upstream processing of recombinant biopharmaceutical products.
Learning Outcomes
On successful completion of this module the learner will be able to:
LO1 Provide an overview of a typical Biopharmaceutical process and discuss the drug discovery and development process.
LO2 Identify potential expression systems for Biopharmaceuticals products.
LO3 Describe the important role of cell banks.
LO4 Discuss media design and sterilisation.
LO5 Describe the main considerations for fermentation vessel design and operation.
LO6 Perform experimental tasks related to upstream processing.
Pre-requisite learning
Module Recommendations
This is prior learning (or a practical skill) that is strongly recommended before enrolment in this module. You may enrol in this module if you have not acquired the recommended learning but you will have considerable difficulty in passing (i.e. achieving the learning outcomes of) the module. While the prior learning is expressed as named CIT module(s) it also allows for learning (in another module or modules) which is equivalent to the learning specified in the named module(s).
Incompatible Modules
These are modules which have learning outcomes that are too similar to the learning outcomes of this module. You may not earn additional credit for the same learning and therefore you may not enrol in this module if you have successfully completed any modules in the incompatible list.
No incompatible modules listed
Co-requisite Modules
No Co-requisite modules listed

This is prior learning (or a practical skill) that is mandatory before enrolment in this module is allowed. You may not enrol on this module if you have not acquired the learning specified in this section.

No requirements listed

Module Content & Assessment

Indicative Content
Biotechnology and the Biopharmaceutical Industry
Identify differences between pharmaceuticals and biopharmaceuticals. Overview of biopharmaceutical therapies and production process.
Drug Discovery and Development
From concept to pharmacies - outline the steps involved in the life history of a successful drug.
Sources of Recombinant Biopharmaceutical Products
An overview of potential expression systems for the production of biopharmaceuticals: microbial systems, animal cells, transgenic animals, plant-based and insect cell culture systems.
Cell Banks
An overview of the production of cell banks, two-tier systems and cryopreservation. Regulatory requirements for purity, identity and stability testing. Inoculum scale up.
Media Design and Sterilisation
Differences and similarities between media for microbiological culture and for mammalian cell culture. Water purity and sterilisation of fermentation media.
Fermenter / Bioreactor Design and Operation
Microbial culture versus mammalian culture. Mode of operation. General design criteria for consideration. Sequence of process steps. Types of vessels.
Laboratory Programme
Conduct laboratory procedures and practical skills employed in upstream processing.
Assessment Breakdown%
Course Work40.00%
End of Module Formal Examination60.00%
Course Work
Assessment Type Assessment Description Outcome addressed % of total Assessment Date
Practical/Skills Evaluation Laboratory practicals and report writing. 3,6 40.0 Every Week
End of Module Formal Examination
Assessment Type Assessment Description Outcome addressed % of total Assessment Date
Formal Exam End-of-Semester Final Examination 1,2,3,4,5 60.0 End-of-Semester
Reassessment Requirement
Repeat examination
Reassessment of this module will consist of a repeat examination. It is possible that there will also be a requirement to be reassessed in a coursework element.

The institute reserves the right to alter the nature and timings of assessment


Module Workload

Workload: Full Time
Workload Type Workload Description Hours Frequency Average Weekly Learner Workload
Lecture Classroom Instruction 2.0 Every Week 2.00
Lab Laboratory Practicals 2.0 Every Week 2.00
Independent & Directed Learning (Non-contact) Self-directed study 3.0 Every Week 3.00
Total Hours 7.00
Total Weekly Learner Workload 7.00
Total Weekly Contact Hours 4.00
This module has no Part Time workload.

Module Resources

Recommended Book Resources
  • Gary Walsh 2007, Pharmaceutical Biotechnology: Concepts and Applications, J. Wiley [ISBN: 9780470012444]
Supplementary Book Resources
  • Brian McNeil & Linda M. Harvey 2008, Practical Fermentation Technology, J. Wiley [ISBN: 9780470014349]
  • Firdos Alam Khan 2015, Biotechnology Fundamentals, CRC Press [ISBN: 9781498723428]
Recommended Article/Paper Resources
  • Obom et al 2013, Operation of a Benchtop Bioreactor, Journal of Visualized Experiments
Other Resources

Module Delivered in

Programme Code Programme Semester Delivery
CR_SPHBI_8 Bachelor of Science (Honours) in Pharmaceutical Biotechnology 4 Mandatory
CR_SBIBI_7 Bachelor of Science in Applied Biosciences and Biotechnology 4 Elective
CR_SFSTE_7 Bachelor of Science in Food and Health Science 4 Elective
CR_SCEBS_8 Common Entry Biological Sciences 4 Elective
CR_SBIOS_6 Higher Certificate in Science in Applied Biosciences 4 Elective

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