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BIOT6002 - Immunoanalysis

Long Title:Immunoanalysis
Module Code:BIOT6002
Duration:1 Semester
Credits: 5
NFQ Level:Fundamental
Field of Study: Biotechnology
Valid From: Semester 1 - 2017/18 ( September 2017 )
Module Delivered in 7 programme(s)
Module Coordinator: Brigid Lucey
Module Author: ANN WARD
Module Description: This module introduces the student to modern immuno-analytical techniques. The concepts and principles of immunochemistry are introduced, as well as the basic principles of biostatistics and quality assurance in the bioanalytical laboratory.
Learning Outcomes
On successful completion of this module the learner will be able to:
LO1 Explain and outline the basic principles of immunochemistry
LO2 Describe and explain the principles of standardisation,optimisation and validation of immunoassay systems
LO3 Outline the elements of quality control and quality assurance in a bioanalytical laboratory
LO4 Use biostatistics to analyse data and solve quantitative problems
LO5 Demonstrate and employ a range of immuno-analytical laboratory skills
Pre-requisite learning
Module Recommendations

This is prior learning (or a practical skill) that is strongly recommended before enrolment in this module. You may enrol in this module if you have not acquired the recommended learning but you will have considerable difficulty in passing (i.e. achieving the learning outcomes of) the module. While the prior learning is expressed as named MTU module(s) it also allows for learning (in another module or modules) which is equivalent to the learning specified in the named module(s).

Incompatible Modules
These are modules which have learning outcomes that are too similar to the learning outcomes of this module. You may not earn additional credit for the same learning and therefore you may not enrol in this module if you have successfully completed any modules in the incompatible list.
No incompatible modules listed
Co-requisite Modules
No Co-requisite modules listed

This is prior learning (or a practical skill) that is mandatory before enrolment in this module is allowed. You may not enrol on this module if you have not acquired the learning specified in this section.

No requirements listed

Module Content & Assessment

Indicative Content
Introduction to Immunochemistry
Structures of antibodies, antibody/antigen interaction, immunoprecipitation, immunodiffusion and immunoelectrophoresis techniques, immunoaffinity chromatography, introduction to immunoassay classification
Immunoassay Techniques
Immunoassay principles & techniques, Particle immunoassays & automation, Optimisation & method validation
Quality assurance and biostatistics
Introduction to the principles of standardisation & IQC in immunoassay, internal and external QA schemes, basic statistical methods & data handling, Control charts and acceptance criteria, reliability and sources of error, Principles of Good Laboratory Practice
Laboratory practice & data analysis
Pipetting skills, immunoprecipitation, optimisation of enzyme linked immunosorbent assay (ELISA), statistical data analysis for validation study
Assessment Breakdown%
Course Work40.00%
End of Module Formal Examination60.00%
Course Work
Assessment Type Assessment Description Outcome addressed % of total Assessment Date
Multiple Choice Questions Assessment of basic principles of immunochemistry 1 10.0 Week 7
Practical/Skills Evaluation Practical reports 4,5 30.0 Every Second Week
End of Module Formal Examination
Assessment Type Assessment Description Outcome addressed % of total Assessment Date
Formal Exam End-of-Semester Final Examination 1,2,3 60.0 End-of-Semester
Reassessment Requirement
Repeat examination
Reassessment of this module will consist of a repeat examination. It is possible that there will also be a requirement to be reassessed in a coursework element.

The institute reserves the right to alter the nature and timings of assessment


Module Workload

Workload: Full Time
Workload Type Workload Description Hours Frequency Average Weekly Learner Workload
Lecture Lecture 2.0 Every Week 2.00
Lab Lab 2.0 Every Second Week 1.00
Independent & Directed Learning (Non-contact) Independent & directed learning 4.0 Every Week 4.00
Total Hours 8.00
Total Weekly Learner Workload 7.00
Total Weekly Contact Hours 3.00
This module has no Part Time workload.

Module Resources

Recommended Book Resources
  • James P. Gosling 2006, Immunoassays, Oxford University Press Oxford [ISBN: 0-19-963711-3]
  • David J Holme & Hazel Peck 2000, Analytical Biochemistry, 3rd Ed., John Wiley & Sons Ltd [ISBN: 9780582294387]
  • Elizabeth Prichard, Vicki Barwick 2007, Quality assurance in analytical chemistry, John Wiley & Sons [ISBN: 0470012048]
Supplementary Book Resources
  • David Wild 2013, The Immunoassay Handbook, 4th Ed., Elsevier Science & Technology [ISBN: 0080970370]
This module does not have any article/paper resources
This module does not have any other resources

Module Delivered in

Programme Code Programme Semester Delivery
CR_SHERB_8 Bachelor of Science (Honours) in Herbal Science 3 Mandatory
CR_SNHSC_8 Bachelor of Science (Honours) in Nutrition and Health Science 3 Mandatory
CR_SPHBI_8 Bachelor of Science (Honours) in Pharmaceutical Biotechnology 3 Mandatory
CR_SBIBI_7 Bachelor of Science in Applied Biosciences and Biotechnology 3 Mandatory
CR_SFSTE_7 Bachelor of Science in Food and Health Science 3 Mandatory
CR_SCEBS_8 Common Entry Biological Sciences 3 Mandatory
CR_SBIOS_6 Higher Certificate in Science in Applied Biosciences 3 Mandatory

Cork Institute of Technology
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Email: help@cit.edu.ie