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BIOT7002 - Bioanalytical Techniques

Title:Bioanalytical Techniques
Long Title:Bioanalytical Techniques
Module Code:BIOT7002
Duration:1 Semester
Credits: 5
NFQ Level:Intermediate
Field of Study: Biotechnology
Valid From: Semester 1 - 2017/18 ( September 2017 )
Module Delivered in 9 programme(s)
Module Coordinator: Brigid Lucey
Module Author: ANN WARD
Module Description: This module aims to advance the student's knowledge of spectroscopic, chromatographic and other current bioanalytical methods.
Learning Outcomes
On successful completion of this module the learner will be able to:
LO1 Describe the fundamental principles of protein purification
LO2 Identify the main analytical applications of electrophoretic separation and current laboratory applications of centrifugation
LO3 Describe the principles of gas chromatography and high performance liquid chromatography(HPLC) separations
LO4 Explain the principle of operation of UV-visible and Infra-red spectroscopic instrumentation
LO5 Use, apply & analyse analytical techniques in the laboratory
Pre-requisite learning
Module Recommendations

This is prior learning (or a practical skill) that is strongly recommended before enrolment in this module. You may enrol in this module if you have not acquired the recommended learning but you will have considerable difficulty in passing (i.e. achieving the learning outcomes of) the module. While the prior learning is expressed as named MTU module(s) it also allows for learning (in another module or modules) which is equivalent to the learning specified in the named module(s).

Incompatible Modules
These are modules which have learning outcomes that are too similar to the learning outcomes of this module. You may not earn additional credit for the same learning and therefore you may not enrol in this module if you have successfully completed any modules in the incompatible list.
No incompatible modules listed
Co-requisite Modules
No Co-requisite modules listed

This is prior learning (or a practical skill) that is mandatory before enrolment in this module is allowed. You may not enrol on this module if you have not acquired the learning specified in this section.

No requirements listed

Module Content & Assessment

Indicative Content
Protein Purification Techniques
Protein precipitation & concentration methods, Size exclusion, ion exchange & affinity chromatography principles & applications.
Applications of centrifugation in analytical laboratories, criteria for design of experimental protocols.
Introduction to principles of separation, overview of electrophoretic techniques, Design & optimisation criteria, Analytical Applications, Analysis & interpretation.
Gas Chromatography and High Performance Liquid Chromatography-instrumentation, mobile and stationary phase, Introduction to GC-Mass Spectrometry
Infra-red Spectroscopy
Origin of absorption bands, instrumentation, sample handling techniques, correlation between infra-red spectra and structural features.
Assessment Breakdown%
Course Work40.00%
End of Module Formal Examination60.00%
Course Work
Assessment Type Assessment Description Outcome addressed % of total Assessment Date
Multiple Choice Questions Assessment of principles of protein purification 1 10.0 Week 7
Practical/Skills Evaluation Performance and submission of reports for laboratory practical experiments 5 30.0 Every Second Week
End of Module Formal Examination
Assessment Type Assessment Description Outcome addressed % of total Assessment Date
Formal Exam End-of-Semester Final Examination 1,2,3,4 60.0 End-of-Semester
Reassessment Requirement
Repeat examination
Reassessment of this module will consist of a repeat examination. It is possible that there will also be a requirement to be reassessed in a coursework element.

The institute reserves the right to alter the nature and timings of assessment


Module Workload

Workload: Full Time
Workload Type Workload Description Hours Frequency Average Weekly Learner Workload
Lecture Lecture 2.0 Every Week 2.00
Lab Lab 2.0 Every Second Week 1.00
Independent & Directed Learning (Non-contact) Independent & Directed Learning 4.0 Every Week 4.00
Total Hours 8.00
Total Weekly Learner Workload 7.00
Total Weekly Contact Hours 3.00
This module has no Part Time workload.

Module Resources

Recommended Book Resources
  • David Sheehan 2009, Physical Biochemistry, 2nd Ed., 2,3,4,5, Wiley-Blackwell England [ISBN: 9780470856031]
  • Wilson & Walker 2010, Principles & Techniques of Practical Biochemistry, 7th Ed., University Press [ISBN: 97805216358]
Supplementary Book Resources
  • Barbara H. Stuart 2004, Infrared spectroscopy, Wiley [ISBN: 0470854286]
  • Graham, J. 2001, Biological Centrifugation, BIOS Scientific Publishers [ISBN: 1-85996-0375]
  • David M. Hawcroft 1996, Electrophoresis, BIOS Scientific Publishers [ISBN: 0199635633]
This module does not have any article/paper resources
Other Resources

Module Delivered in

Programme Code Programme Semester Delivery
CR_SAGBI_8 Bachelor of Science (Honours) in Agri-Biosciences 4 Mandatory
CR_SHERB_8 Bachelor of Science (Honours) in Herbal Science 4 Mandatory
CR_SNHSC_8 Bachelor of Science (Honours) in Nutrition and Health Science 4 Mandatory
CR_SPHBI_8 Bachelor of Science (Honours) in Pharmaceutical Biotechnology 4 Mandatory
CR_SAGBI_7 Bachelor of Science in Agri-Biosciences 4 Mandatory
CR_SBIBI_7 Bachelor of Science in Applied Biosciences and Biotechnology 4 Mandatory
CR_SFSTE_7 Bachelor of Science in Food and Health Science 4 Mandatory
CR_SCEBS_8 Common Entry Biological Sciences 4 Mandatory
CR_SBIOS_6 Higher Certificate in Science in Applied Biosciences 4 Mandatory

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