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BIOL6017 - Metabolic Biochemistry

Title:Metabolic Biochemistry
Long Title:Metabolic Biochemistry
Module Code:BIOL6017
Duration:1 Semester
Credits: 5
NFQ Level:Fundamental
Field of Study: Biochemistry & Cell Biology
Valid From: Semester 1 - 2017/18 ( September 2017 )
Module Delivered in 9 programme(s)
Module Coordinator: Brigid Lucey
Module Author: Fiona OHalloran
Module Description: This module teaches the principles of enzyme catalysis, inhibition and regulation, leading on to a study of selected metabolic pathways and their integration and control.
Learning Outcomes
On successful completion of this module the learner will be able to:
LO1 Explain the principles of enzyme catalysis, inhibition and regulation.
LO2 Design and optimise assays to monitor enzyme reactions and assess reaction rates.
LO3 Outline selected metabolic pathways and describe the principles that govern their regulation
LO4 Perform laboratory experiments to demonstrate enzyme action and quantify reaction rate constants.
LO5 Write scientific reports to analyse and interpret experimental data
Pre-requisite learning
Module Recommendations

This is prior learning (or a practical skill) that is strongly recommended before enrolment in this module. You may enrol in this module if you have not acquired the recommended learning but you will have considerable difficulty in passing (i.e. achieving the learning outcomes of) the module. While the prior learning is expressed as named MTU module(s) it also allows for learning (in another module or modules) which is equivalent to the learning specified in the named module(s).

12751 BIOL6017 Metabolic Biochemistry
Incompatible Modules
These are modules which have learning outcomes that are too similar to the learning outcomes of this module. You may not earn additional credit for the same learning and therefore you may not enrol in this module if you have successfully completed any modules in the incompatible list.
No incompatible modules listed
Co-requisite Modules
No Co-requisite modules listed

This is prior learning (or a practical skill) that is mandatory before enrolment in this module is allowed. You may not enrol on this module if you have not acquired the learning specified in this section.

No requirements listed

Module Content & Assessment

Indicative Content
Biological functions of enzymes / Energy of activation and catalysis / Classification of enzymes / Enzyme-substrate complex / Kinetics (Michaelis-Menton, Km, Vmax, Ki, Lineweaver Burk) / Temperature and pH effects / Regulation of enzyme activity / Enzyme inhibition / Units of enzyme activity / Introduction to enzyme assay design (monitoring assays, coupling reactions, fixed time assays and kinetic assays).
Cellular energetics and ATP
Biological redox reactions, reduction potential/energy coupling reactions, ATP hydrolysis.
Carbohydrate metabolism
Overview of relevant metabolic pathways (glycolysis, citric acid cycle, electron transport chain) / substrate level and oxidative phosphorylation / metabolic regulation and control points.
Overview of metabolic pathway / Cori cycle / metabolic control points / hormonal regulation
Lipid metabolism
Overview of Fatty acid metabolism / beta oxidation / metabolic regulation.
Urea cycle
Amino acid metabolism / Ammonia formation / overview of the urea cycle / metabolic regulation.
Practical skills
Methods for quantitation of biomolecules / determination of Km and Vmax using fixed time and continuous monitoring methods / effect of temperature, pH and reactant concentrations on enzyme reaction rates / estimation of precision and accuracy of experimental results.
Assessment Breakdown%
Course Work100.00%
Course Work
Assessment Type Assessment Description Outcome addressed % of total Assessment Date
Multiple Choice Questions Theory-based assessment of lecture material 1,2 25.0 Week 7
Short Answer Questions Theory-based assessment of lecture material 2,3 35.0 Week 13
Written Report Themed lab reports and data handling 4,5 20.0 Every Second Week
Practical/Skills Evaluation Laboratory exam 4,5 20.0 Week 12
No End of Module Formal Examination
Reassessment Requirement
Repeat examination
Reassessment of this module will consist of a repeat examination. It is possible that there will also be a requirement to be reassessed in a coursework element.

The institute reserves the right to alter the nature and timings of assessment


Module Workload

Workload: Full Time
Workload Type Workload Description Hours Frequency Average Weekly Learner Workload
Lecture class-based instruction 2.0 Every Week 2.00
Lab practical laboratory instruction 2.0 Every Week 2.00
Independent & Directed Learning (Non-contact) study of lecture notes 2.0 Every Week 2.00
Independent & Directed Learning (Non-contact) report preparation, data handling exercises 1.0 Every Week 1.00
Total Hours 7.00
Total Weekly Learner Workload 7.00
Total Weekly Contact Hours 4.00
This module has no Part Time workload.

Module Resources

Recommended Book Resources
  • Nelson, D.L. & Cox, M. M. 2017, Lehninger principles of biochemistry, 7th Ed., Freeman and Sapling [ISBN: 9781464126116]
Supplementary Book Resources
  • Berg, J.M., Tymoczko, J.L., Gatto, G.J. & Stryer, L. 2015, Biochemistry, 8th Ed., W.H. Freeman [ISBN: 9781464126109]
  • Moorthy, K. 2007, Fundamentals of Biochemical Calculations, 2nd Ed., CRC Press [ISBN: 1420053574]
  • Dwivedi, Neeraja, Dwivedi, Prakash 2009, Comprehensive Biochemistry, Ebook, Pragati Prakashan [ISBN: 9789350434536]
This module does not have any article/paper resources
Other Resources

Module Delivered in

Programme Code Programme Semester Delivery
CR_SAGBI_8 Bachelor of Science (Honours) in Agri-Biosciences 4 Mandatory
CR_SHERB_8 Bachelor of Science (Honours) in Herbal Science 4 Mandatory
CR_SNHSC_8 Bachelor of Science (Honours) in Nutrition and Health Science 4 Mandatory
CR_SPHBI_8 Bachelor of Science (Honours) in Pharmaceutical Biotechnology 4 Mandatory
CR_SAGBI_7 Bachelor of Science in Agri-Biosciences 4 Mandatory
CR_SBIBI_7 Bachelor of Science in Applied Biosciences and Biotechnology 4 Mandatory
CR_SFSTE_7 Bachelor of Science in Food and Health Science 4 Mandatory
CR_SCEBS_8 Common Entry Biological Sciences 4 Mandatory
CR_SBIOS_6 Higher Certificate in Science in Applied Biosciences 4 Mandatory

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